Table 1.
Primer sequences and annealing temperatures (Tm)
| Gene | Sequence | Tm (°C) | GenBank Accession |
|---|---|---|---|
| COX-1 | F 5'-ATGAGTACCGCAAGAGGTTTGG-3' R 5'-ACGTGGAAGGAGACATAGG-3' | 57 | NM_001009476 |
| COX-2 | F 5'-CAGAGCTCTTCCTCCTGTGC-3' R 5'-CAAAAGGCGACGGTTATGC-3' | 58 | NM_001009432 |
| Collagen Iα1 | F 5'-AAGACATCCCACCAGTCACC-3' R 5'-CAGATCACGTCATCGCACA-3' | 60 | AF129287 |
| Collagen Iα2 | F 5'-GGCTCAACCTGAAGACATCC-3' R 5'-TCTCCTACCCAGACATGCTTC-3' | 59 | AF129287 |
| Collagen III | F 5'-CTGCTGGAAAGAATGGTGAG-3' R 5'-GTCACCAGAAGGCCCAGTA-3' | 58 | AF129287 |
| eNOS | F 5'-GATCAGCAACGCTATCACGA-3' R 5'-ATACGGCTTGTCACCTCCTG-3' | 54 | NM_001129901 |
| Tropoelastin | F 5'-ATCTCTCAGTCAGGCACCAG-3' R 5'-GTTTGTTGGGAAAGAAAGCA-3' | 54 | NM_175772 |
| β-Actin | F 5'-CATCCTGACCCTCAAGTACCC-3' R 5'-GTGGTGGTGAAGCTGTAGCC-3' | 58 | U39357 |