Table 1.

Primer sequences

Gene	Direction and sequence
IL-1β	F:5′-TCGTGCTGTCGGACCCATAT-3′ R: 5′-GTCGTTGCTTGGTTCTCCTTGT-3′
TNF-α	F: 5′-GCGGAGTCCGGGCAGGTCTA-3′ R: 5′-GGGGGCTGGCTCTGTGAGGA-3′
ICAM-1	F: 5′-GCGGAGTCCGGGCAGGTCTA-3′ R: 5′-GGGGGCTGGCTCTGTGAGGA-3′
MCP-1	F:5′-ACCACAGTCCATGCCATCAC-3′ R: 5′-TTGAGGTGGTTGTGGAAAAG-3′
GAPDH	F: 5′-CCCAGCAAGGACACTGAGCAAG-3′ R: 5′-GGTCTGGGATGGAAATTGTGAGGG-3′

IL-1β, interleukin-1β; TNF-α, tumour necrosis factor-α; ICAM-1, intracellular cell adhesion molecule-1; MCP-1, monocyte chemotactic protein-1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.