Skip to main content
. 2014 Jun 10;142(3):384–395. doi: 10.1111/imm.12261

Figure 1.

Figure 1

Analysis of global DNA methylation in 5-aza-2′-deoxycytidine (5-Aza-dC) and epigallocatechin-3-gallate (EGCG) cultured CD4+ CD25− T cells. Cells were cultured with two different concentrations of 5-Aza-dC and EGCG and stimulated with anti-CD3 and anti-CD28 monoclonal antibody before cultivation. DNA methylation was normalized to anti-CD3 and anti-CD28 stimulated CD4+ CD25− T cells, not treated with hypomethylating agents. The DNA methylation of these cells was set 100%. *P < 0·05, **P < 0·01, ***P < 0·001.