Figure 6.

Suppression assay. 5-aza-2′-deoxycytidine (5-Aza-dC) and epigallocatechin-3-gallate (EGCG) stimulated CD4⁺ CD25⁻ T cells were co-cultured with responder T cells (stained with eFluor670 proliferation dye) and dendritic cells in the presence of CD3 monoclonal antibody for 3 days and analysed by flow cytometry. Unstimulated CD4⁺ CD25⁻ T cells co-cultured with responder T cells were used as control population. Degree of inhibition was determined by measuring proliferative capacity of cultured cells compared with the control population.