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. 2014 Jun 10;142(3):431–441. doi: 10.1111/imm.12275

Figure 1.

Figure 1

Genetic engineering of mesenchymal stromal cells (MSC)s with a central nervous system (CNS) -targeting receptor. (a) Anti- myelin oligodendrocyte glycoprotein (MOG) antibody staining of 293T cells transduced with Lenti-murine MOG. (b) MSCs transduced with CARαMOG receptors were analysed for surface expression using flow cytometry. (c) MSCs and engineered MSCs were mixed with αCD3/interleukin-2-stimulated T cells at a 1 : 2 ratio and analysed for suppressive ability in a thymidine-based assay. All samples were analysed in triplicates. Engineered MSCs suppressed activated T cells (P < 0·001). Engineered MSCs retained their suppressive function in the presence of lipopolysaccharide-activated macrophages (P < 0·001) or MOG-expressing cells (P < 0·001). All experiments in this figure were repeated at least three times with similar results. Statistics were analysed using the Mann–Whitney test and GraphPad prism software.