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. 2014 Jun 10;142(3):431–441. doi: 10.1111/imm.12275

Figure 5.

Figure 5

Immunological evaluation of intranasal mesenchymal stromal cell (MSC) treatment efficacy. T cells from brain (a, n = 3) and spleen (b, n = 3) from MSC CARαMOG and MSC Mock-treated experimental autoimmune encephalomyelitisEAE mice were isolated using a MACS T-cell separation kit, stimulated for 24 hr and analysed for cytokine production [interleukin-17 (IL-17) and interferon-γ (IFN-γ)]. T cells isolated from brain were stimulated with myelin oligodendrocyte glycoprotein (MOG) peptides (MOG37–46, MOG35–55) whereas T cells isolated from the spleen were stimulated with αCD3/IL-2. In the brain CD4+ T cells responded by secreting IL17 to a higher extent in the MSC Mock-treated mice compared with MSC CARαMOG-treated mice; however, the difference was not significant. Effector cytokines (c: IFN-γ, d: IL-12) were analysed by quantitative PCR from brain biopsies obtained from MSC CARαMOG-treated and MSC Mock-treated mice (15 days post-MSC treatment).