Figure 1.

Phenotypic characterization of isolated T cells. Plots display the fractions of CD4⁺ T cells in reference to CD25 expression, CD8⁺ T cells and B lymphocytes before isolation (left panels). Isolation CD4⁺ CD25⁻ T cells yields low contamination with CD4⁺ CD25⁺ T cells and CD8⁺ T cells (middle panels). The CD4⁺ CD25⁺ subset contains ∼ 10% CD4⁺ CD25⁻ T cells and ∼ 85% express FoxP3 (right panels).