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. 2014 Jun 10;171(13):3158–3170. doi: 10.1111/bph.12654

Figure 2.

Figure 2

Integrin β3 mediated Src phosphorylation induced by hypotonic solution. (A) Phospho-Src (pY416) stimulated by hypotonic solution from 0 to 60 s. (i) Representative Western blot is shown. (ii) Densitometric analysis shows that phospho-Src (pY416) induced by hypotonic solution was time dependent (*P < 0.05 vs. isotonic (Iso) group, **P < 0.01 vs. Iso group; data from five different experiments). (B) Effect of integrin β3 cDNA transfection on phospho-Src (pY416). (i) Representative Western blot. (ii) Densitometric analysis. (*P < 0.05 vs. Iso group, #P < 0.05 vs. vector group; data from five different experiments). (C) Effect of integrin β3 siRNA transfection on phospho-Src (pY416) induced by hypotonic solution. (i) Representative Western blot. (ii) Densitometric analysis. (*P < 0.05 vs. Iso group, #P < 0.05 vs. negative group; data from five different experiments). Iso, isotonic solution; Hypo, hypotonic solution.