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. 2014 Jun 10;171(13):3182–3195. doi: 10.1111/bph.12659

Figure 2.

Figure 2

Reactive oxygen species generation (ROS) and mitochondrial depolarization are involved but not required in ABT-263 and sorafenib combination-induced apoptosis. (A) BEL7402 cells were treated with ABT-263 and sorafenib either alone or in combination for 48 h and the intracellular ROS levels assessed. (B) BEL7402 cell viability was determined using the trypan blue exclusion assay in either the presence or absence of 15 mM NAC for 48 h. (C) BEL7402 cell viability was measured by trypan blue exclusion assay in either the presence or absence of 15 mM Tiron for 48 h. (D) To evaluate the time course of the mitochondrial membrane potential, BEL7402 cells were treated with ABT-263 and sorafenib either alone or in combination for 48 h followed by DCF fluorescence analysis. (E) BEL7402 cell viability was determined using the trypan blue exclusion assay in either the presence or absence of 2 μM CsA for 48 h.