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. 2014 Jun 10;171(13):3196–3211. doi: 10.1111/bph.12661

Figure 7.

Figure 7

hMOF regulates the expression of drug-defence genes. (A–C) hMOF knockdown reduces expression of self-defence genes. A549 cells were transfected with NC-siRNA or hMOF-siRNA for 24 h, followed by 5 μΜ CDDP treatment for 48 h. Total RNA was subjected to q-PCR analysis for the indicated antioxidant genes (A), drug metabolising enzymes (B) and ATP-dependent drug efflux pumps (C). (D) hMOF knockdown reduces glutathione production. The intracellular total glutathione of the cells in (A) was quantified. (E) CDPP enhances Nrf2 interaction with hMOF. (F) hMOF knockdown reduces CDDP-induced acetylation of Nrf2. The total proteins in (A) were subjected to IP and IB analysis with the indicated antibodies. *P < 0.05, **P < 0.01; significantly different fromNC-siRNA; ##P < 0.01, significantly different from NC-siRNA + CDDP.