Figure 3.

Silencing UCP2 with adenovirus ucp2 short hairpin RNA reduced the UCP2 expression (A), elevated nitrotyrosine (N-tyr) level (B) and abolished the ability of berberine (BBR) to suppress N-tyr contents (B) in transfected INS-1E cells as compared with scramble control. (C) Increased Ang II-stimulated mitochondrial ROS production following 8 h exposure to HG (30 mmol·L⁻¹) was reversed by BBR (5 μmol·L⁻¹), whereas the effect of BBR was inhibited by Compound C (CC; 10 μmol·L⁻¹) or genipin (1 μmol·L⁻¹). (D) Silencing UCP2 with adenovirus ucp2 short hairpin RNA increased Ang II-stimulated mitochondrial ROS production compared with scramble control. HG-induced further elevation in mitochondrial ROS production in transfected INS-1E cells was unaffected by berberine (5 μmol·L⁻¹). Results are means ± SEM of four to six experiments. *P < 0.001 versus control or control (scramble); #P < 0.001 versus HG or HG (scramble); †P < 0.001 versus control (shUCP2) or HG + BBR.