Table 1.

CI of PI103 and Ku-0063794 in GC cells grown in LG and HG conditions

Cell line	Glucose	Single-agent IC50 (μM)		IC50 (μM) in combination		CI at fu 0.5
		PI103	Ku-0063794	PI103	Ku-0063794
AGS	High	0.52 ± 0.08	0.72 ± 0.05	0.68 ± 0.031	0.87 ± 0.031	1.1 ± 0.020
	Low	2.57 ± 0.04	15.0 ± 2.91	0.05 ± 0.001*	0.03 ± 0.003*	0.03 ± 0.001**
HGC27	High	0.49 ± 0.01	1.33 ± 0.03	0.38 ± 0.022	0.93 ± 0.022	0.97 ± 0.012
	Low	15.0 ± 0.50	15.0 ± 4.82	0.02 ± 0.004*	0.05 ± 0.004*	0.01 ± 0.003**
MKN45	High	1.21 ± 0.03	1.01 ± 0.02	1.01 ± 0.051	0.86 ± 0.067	0.93 ± 0.340
	Low	0.92 ± 0.06	0.82 ± 0.01	0.87 ± 0.030	1.2 ± 0.052	1.0 ± 0.223
NUGC4	High	16.0 ± 1.30	3.51 ± 0.19	14.0 ± 3.913	13.0 ± 1.627	0.89 ± 0.320
	Low	15.0 ± 2.12	2.93 ± 0.31	14.0 ± 5.321	16.0 ± 4.963	0.85 ± 0.671

The IC₅₀ values of PI103 and Ku-0063794 as single agents and in combination (in the presence of the other drug at fixed, non-growth inhibitory concentrations of one-fifth IC₅₀) are indicated. CI values for fu at 0.5 (IC₅₀) are also given. Additivity = 1, antagonism > 1, synergy < 1.

^*P < 0.05 for differences in IC₅₀ values between LG and HG values.

^**P < 0.05 for differences from unity for CI values.