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. 2014 Jun 25;7:290. doi: 10.1186/1756-3305-7-290

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Copyright © 2014 McWilliam et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of native Sj-L6L-1 in S. japonicum extracts. A: Protein extracts from S. japonicum cercariae (C), schistosomula (S), adult worm pairs (A), or separated male (M) or female (F) worms were analysed by SDS-PAGE and stained with coomassie (lanes 2–6) or subjected to immunoblotting (lanes 7–11) with antisera to rSj-L6L-1. Molecular weight (MW) markers are shown in lane 1. B: Adult worms were fractionated into tegument (teg), aqueous-soluble (sol) and -insoluble (ins) worm fractions, and analysed by immunoblotting with antisera to rSj-L6L-1.