Table 3. Primers used for molecular diagnostics.
| Species | Target | Forward primer Sequence (5′→3′) | Reverse primer Sequence (5′→3′) | Probe Sequence (5′→3′) | Size (bp) | Reference |
| Streptomyces spp. | 16S rRNA | AGAGTTTGATMTGGCTCAG | AAGGAGGTGWTCCARCC | 1363 to 1521 | [28] | |
| Nocardia spp. | 16S rRNA | ACCGACCACAAGGGG | GGTTGTAACCTCTTCGA | 596 | [54] | |
| Nocardia spp. | 16S rRNA | GCTTAACACATGCAAGTCG | GAATTCCAGTCTCCCCTG | 606 | [31] | |
| Nocardia spp. | 16S rRNA | AGAGTTTGATCCTGGCTCAG | ATTACCGCGGCTGCTGG | 498 | [59] | |
| Nocardia spp. | 16S-23S rRNA intergenic spacer | GTGCGGCTGGATCACCTCCT | GACAGCTCCCCGAGGCTTATCGCA | various | [58] | |
| Nocardia spp. | hsp65 | ACCAACGATGGTGTGTCCAT | CTTGTCGAACCGCATACCCT | 441 | [55], [59] | |
| Nocardia spp. | hsp65 | GTTGTCCTGGAGAAGAAGTGG | CTTGTCGAACCGCATACCCT | 476 | [59] | |
| Nocardia spp. | gyrB | CTTCGCCAACACCATCAACAC | TGATGATCGACTGGACCTCG | 610 | [59] | |
| Nocardia spp. | gyrB | CGAGGAGGGCTTCCGCGCGG | ATCGACTGGACCTCGTTGTTC | 521 | [59] | |
| Nocardia spp. | secA1 | GCGACGCCGAGTGGATGG | TTGGCCTTGATGGCGTTGTTC | 483 | [59] | |
| Nocardia spp. | secA1 | GCGACGCCGAGTGGATGG | GCGGACGATGTAGTCCTTGTC | 520 | [59] | |
| Nocardia spp. | rpoB | CCGCTACAAGATCAACAAGAAGC | CCCGCGAGGACATCGTCG | 737 | [59] | |
| Nocardia spp. | rpoB | CCGCTACAAGATCAACAAGAAGC | GGCGACGTACTCCATCTCCTC | 780 | [59] | |
| Nocardia spp. | rpoB | CCCGCGAGGACATCGTCG | CCCGCGAGGACATCGTCG | 655 | [59] | |
| Nocardia spp. | rpoB | CGAGTACCTGGTGCGYCTGC | TCGACCGGCGAGTTGGCCTG | 599 | [59] | |
| Fungi | ITS | TTACGTCCCTGCCCTTTGTA | GCATTCCCAAACAACTCGACTC | Various | [64] | |
| Fungi | ITS | TCCTCCGCTTATTGATATGC | GGAAGTAAAAGTCGTAACAAGG | Various | [67] | |
| M. mycetomatis | ITS | AATGAGTTGGGCTTTAACGG | TCCCGGTAGTGTAGTGTCCCT | 420 | [67] | |
| Sc. boydii | ITS | GAGGCAATAACAGGTCTGTGATGC | TTACTACGCAGAAGGCAA | 800 | [68] | |
| Sc. boydii | ITS | AATCTTTGAACGCACATTG | TTACTACGCAGAAGGCAA | 197 | [24] | |
| Sc.boydii | ITS | TGTCCGAGCGTCATTTC | TTACTACGCAGAAGGCAA | 149 | [24] | |
| Sc. boydii | ITS | ATGGGCACCGAAGAAGCA | CGCGCAGACACGATA | p GGGTCGCGAAGACTCGCCGTAgatcaTGCTTCTTCGGTGCCCATtaccggtgcggatagctacCGCGCAGACACGATAgtctaTTTCAGGGCCTACGGA | Various* | [70] |
| Sc.boydii | BT2 | TGGCGAGCACGGTCTTG | ACATTCACGGCAGACACTGATT | FAM-TAGCAACGGAGTGTACGGAACCACCC-BBQ | 96** | [69] |
* Rolling Circle amplification assay, probe listed is the padlock probe to be used in this assay.
** quantitative real time PCR assay.