Table 2. Differentially expressed genes in neurotransmitter receptors by Toxoplasma infection.
| Category | Gene name | Microarray | qPCR | Protein | |||
|---|---|---|---|---|---|---|---|
|
|
|
|
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| FCa | Pb | FC | P | FC | P | ||
| Type I | |||||||
| Dopaminergic | DRD1c (Dopamine receptor D1) | -1.27 | 8.18E-03 | -1.42 | 0.015 | -2.79 | 0.001 |
| Glutamatergic | GRIN2A (Glutamate receptor, N-methyl D-aspartate 2A) | 1.29 | 8.24E-03 | -1.47 | 0.018 | -1.93 | 0.048 |
| Serotonergic | HTR1D (Serotonin receptor 1D) | 1.39 | 8.46E-03 | -1.51 | 0.038 | _d | /e |
| HTR3E (Serotonin receptor 3, family member E) | 1.24 | 3.95E-03 | -1.52 | 0.046 | -1.59 | 0.041 | |
| Type III | |||||||
| Glutamatergic | GRIN2A (Glutamate receptor, N-methyl D-aspartate 2A) | 1.46 | 6.09E-04 | -1.67 | NSf | / | / |
| Adrenergic | ADRB3 (Adrenergic, beta-3-, receptor) | 1.32 | 7.10E-04 | -1.55 | NS | / | / |
| ADRA1A (Adrenergic, alpha-1A-, receptor) | -1.49 | 7.32E-03 | _ | / | / | / | |
a
Fold changes were calculated by comparison of the mean expression value in the infection group to the control group. If the fold-change is less than 1, the value was replaced by the negative of its inverse.
b
P-values were calculated using Student's t-test for Toxoplasma infection and control. A mixed regression model was used to analyze repeated-measures.
c
genes with similar expression pattern at qPCR and protein level are indicated with bold font.
d
_: inconclusive result.
e
/: not applicable.
f
NS: not significant. In the analysis of qPCR/Western blot data, all genes/proteins were normalized to the endogenous control gene β-actin.