Figure 2.

CAR expression on primary human fibroblasts. Twenty-four hours after infection with AdCAR, AdNull (both 10⁴ pu/cell), or no virus (naive), fibroblasts were incubated with anti-CAR antibody (RmcB) or isotype-matched control antibody followed by Texas red–labeled secondary antibody and examined by fluorescent microscopy. Naive A549 cells served as positive control. (a) A549, RmcB. (b) A549, control antibody. (c) Naive fibroblasts, RmcB. (d) AdNull-infected fibroblasts, RmcB. (e) AdCAR-infected fibroblasts, RmcB. (f) AdCAR-infected fibroblasts, control antibody. Scale bar, 10 μm. CAR, Coxsackie virus and adenovirus receptor.