Figure 4.

Binding of fluorescent Ad vectors by CAR-sufficient primary fibroblasts. AdCAR- infected fibroblasts were incubated with Cy3-labeled fluorescent AdGFP (10¹¹ pu/cell) for 10 min at 37°C. The cells were then fixed and stained for CAR as described for Fig. 2, but with FITC-labeled secondary antibody. Naive A549 cells served as positive control. (a) A549 cells, DIC. (b) A549 cells, Cy3-AdGFP binding. (c) A549 cells, RmcB. (d) AdCAR-infected fibroblasts, DIC. (e) AdCAR-infected fibroblasts, Cy3-Ad5GFP binding. (f) AdCAR-infected fibroblasts, RmcB. Scale bar, 10 μm. DIC, differential interference contrast.