Figure 9.
βgal gene transfer and expression mediated by an Ad vector that lacks the penton base RGD domain in CAR-sufficient versus CAR-deficient fibroblasts. Mock-, AdNull-, or AdCAR- infected fibroblasts were infected with 300 pu/cell of Adβgal (+RGD, an Ad vector containing a normal penton base RGD sequence) or AdΔRGDβgal (ΔRGD, an Ad vector lacking the penton base RGD sequence). βgal activity of cell lysates was assayed 24 h after the second infection. The dashed line indicates limit of detection of βgal assay (103 relative light units/mg protein). The data are presented as mean ± SE of triplicate measurements.