Figure 5.

Ara h 3 epitopes can be mutated to non–IgE-binding peptides. Epitope 4 was synthesized with an alanine residue substituted for one of the amino acids at each position in the peptide. The synthesized peptides were probed with a pool of serum IgE from peanut-hypersensitive patients whose IgE has previously been shown to recognize this peptide. The letters to the right of each lane indicate the one-letter amino acid code for the residue normally at that position and the amino acid substituted for that residue. The numbers indicate the position of each residue in the Ara h 3 protein. WT indicates the wild-type peptide with no amino acid substitutions.