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. 2014 May 9;42(12):e100. doi: 10.1093/nar/gku381

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Overview of constructed strains and 70S ribosome structure. (A) Given are the names of the constructed strains as used in this study (lab nomenclature in brackets), relevant genotype and fluorescent fusion proteins produced. pTRC-rpsQ, pTRC-rplC: complementation plasmids with copies of the chromosomally deleted genes. Endogenous genes are shown as gray arrows, genes encoding FPs as colored boxes. Genes to be deleted were replaced by kanamycin resistance cassettes (KanR). mCherry gene and protein portions are shown in red, EGFP accordingly in green. (B) Surface representation of an E. coli 70S ribosome crystal structure. The 16S rRNA is colored in light gray proteins of the small subunit in yellow. 23S and 5S rRNA are shown in dark gray, proteins of the large subunit in cyan. S2 is highlighted in red, L19 in green. Their surface exposed C-termini are shown in purple. The figure was generated with pymol, based on PDB files 3R8S and 4GD1 (28).