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. 1999 Feb 15;103(4):497–505. doi: 10.1172/JCI5542

Figure 2.

Figure 2

Targeted disruption of the GalNAcT locus and generation of Hexb–/– GalNAcT–/– double knockout mice. (a) The structure of the mouse GalNAcT targeting vector is on the top, the structure of the mouse GalNAcT locus is in the middle, and the predicted structure of the homologously recombined locus is on the bottom. (b) Genotyping of mouse offspring from the Hexb+/– GalNAcT+/– double heterozygous mating. Genomic DNA was isolated from mouse tails, digested with HindIII endonuclease, electrophoresed on a 1% agarose gel, and transferred to a nylon membrane. The hybridization was performed with both the Hexb probe (11) and GalNAcT probe simultaneously, a. Wild-type Hexb locus yielded a 3-kb HindIII band. Disrupted Hexb locus yielded a 4-kb HindIII band. Wild-type GalNAcT locus showed an 8-kb HindIII band. Targeted GalNAcT locus showed a 10-kb HindIII band. (c) TLC analysis of acidic (a; lanes 1 and 3) and neutral (n; lanes 2 and 4) lipid fractions from brains of GalNAcT+/+ mice and GalNAcT–/– mice.