Figure 8.

Binding of hAPE1 to an AP site in duplex or quadruplex DNA structure. Electrophoretic mobility shift assays were carried out to analyse binding of hAPE1 to DNA probes consisting of double-stranded substrates containing an AP site at position 12 of the c-myc G repeat. ds-c-myc-AP12 was incubated in the presence or absence of PEG and KCl to induce G4 formation, followed by incubation with hAPE1 in binding buffer at room temperature in the presence or absence or competitor DNA. Competitor DNA consisted of identical sequence in duplex or quadruplex DNA conformation. After incubation the binding reaction was immediately loaded on a 6% non-denaturing polyacrylamide gel.