Table 1.
T7 primer |
5'-TAATACGACTCACTATA-3' |
c-myc-WT |
5'-gctacatgctctagatct/ggaaggggtgggaggggtgggaggggt/CTG |
CACccagctgcttcgtccgaagaccctatagtgagtcgtatta-3' |
c-myc-A12 |
5'-gctacatgctctagatct/ggaaggggtgggaggAgtgggaggggt/CTG |
CACccagctgcttcgtccgaagaccctatagtgagtcgtatta-3' |
c-myc-AP12 |
5'-gctacatgctctagatct/ggaaggggtgggaggXapgtgggaggggt/CTG |
CACccagctgcttcgtccgaagaccctatagtgagtcgtatta-3' |
Sequences delimited by (/) correspond to wild type (WT) and mutant sequences from the c-myc NHEIII1; sequences shown in italic correspond to the T7 RNA polymerase promoter; Xap, apurinic (abasic) site.