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. 2014 May 14;42(12):7720–7733. doi: 10.1093/nar/gku421

Figure 5.

Figure 5.

53BP1 IRIF can be restored in RNF8 depleted cells by expression of RNF168 WT and N221*. U2OS cells were treated with siCTRL or siBRCA1 and siRNF8 and subsequently transfected with a set of the Flag-tagged RNF168 expression vectors or the RNF8 expression vector, which are described in Figures 2A and 4A. Subsequently, cells were treated with 6 Gy of IR (Cs137), and allowed to recover for 4 h prior to fixation and immunostaining. RNF8 and 53BP1 antibodies were used for the RNF8 expression vector samples, and Flag and 53BP1 antibodies were used for the RNF168 expression vector samples. Shown are immunostaining, and DAPI staining images for representative cells from each transfection. Scale bar = 10 μm.