Table 1.

Sequences of I-SceI-resistant Proximal-EJ products amplified from the 3′ I-SceI site in the EJ5-GFP reporter, following the CAS9-WT and Trex2 transfections described in Figure 1C

I-SceI site in capital letters. / = predicted CAS9 cleavage site. (deleted nucleotides). bold = mutation	Incidence
Parental sequence
agctTAGGGATAACAGGG/TAATggatc
CAS9-WT junctions
agctTAGGGATAAC(A)GGG/TAATggatcc	1/14
agctTAGGGATAACAG(GG)/TAATggatcc	1/14
agctTAGGGATAACA(GGG)/TAATggatc	4/14
agctTAGGGATAACAGGG/(TAATgg)atcc	1/14
agctTAGGGATAACAGGG/(TAATGG)atcc	1/14
agctTA(GGGATAACAGGG/TA)Atggatcc	2/14
agctTAGGGACAA(CAGGG/TAATggatcc)ac	1/14
ag(c)tTA(GGGATAACAGGG/TAATg)gatcc	1/14
agctA(AGGGATAACAGGG/TAATg)gatcc	1/14
cca(tcagaagctTAGGG/ATAACAGGGTAATggatcca)ccggt	1/14
CAS9-WT + Trex2 junctions
agctTAGGGATAACAG(GG/)TAATggatcc	1/11
agctTAGGGATAACAGGG/(TAATgg)atcc	1/11
agctTAGGGATAAC(AGGG/TAA)Tggatcc	1/11
agctTAGGGATAAC(AGGG/TAAT)ggatcc	1/11
agctTAGGGA(TAACAGGG/)TAATggatcc	1/11
agctTAGGG(ATAACAGGG/T)AATggatcc	1/11
agctT(AGGGATAACAGGG/)TAATggatcc	1/11
agctTA(GGGATAACAGGG/TA)Atggatcc	1/11
agctTAGG(GATAACAGGG/TAATgg)atcc	1/11
ttttg(54nt)tcgccgccat	1/11
ttcta(56nt)gtgagc	1/11