Table 2.

Quantification of IRIF in experiments described in Figures 3, 5 and 6

siRNA treatment and RNF168 expression	Frequency of FLAG IRIF of FLAG+ cells		Frequency of 53BP1 IRIF of FLAG+ cells
	#>5 IRIF/ total (%)	Distinct from WT	#>5 IRIF / total (%)	Distinct from WT	Distinct from N221*
siRNF168 + siBRCA1
Flag-RNF168-WT	124/158 (79)		119/158 (75)
Flag-RNF168-R57D	25/156 (16)	P < 0.0001	1/156 (0.6)	P < 0.0001	P < 0.0001
Flag-RNF168-N221*	0/188 (0)	P < 0.0001	140/188 (75)
Flag-RNF168-N221*/R57D	0/175 (0)	P < 0.0001	1/175 (0.6)	P < 0.0001	P < 0.0001
Flag-RNF168-N221-571	0/95 (0)	P < 0.0001	0/95 (0)	P < 0.0001	P < 0.0001
Flag-RNF168-N221* LRM1Δ	0/86 (0)	P < 0.0001	67/86 (78)
Flag-RNF168-N221* LLAA-A179G	0/89 (0)	P < 0.0001	76/89 (85)		P = 0.044
Flag-RNF168-N221* LRM1Δ-LLAA-A179G	0/93 (0)	P < 0.0001	49/93 (53)	P = 0.0003	P < 0.0001
siRNF8 + siBRCA1
Flag-RNF168-WT	94/117 (90)		92/117 (79)		P < 0.0001
Flag-RNF168-R57D	15/114 (13)	P < 0.0001	0/114 (0)	P < 0.0001	P < 0.0001
Flag-RNF168-N221*	2/113 (2)	P < 0.0001	59/113 (52)	P < 0.0001
Flag-RNF168-N221*/R57D	0/114 (0)	P < 0.0001	4/114 (3.5)	P < 0.0001	P < 0.0001

For each condition, cells were accumulated from immunofluorescence staining of at least three independent transfections. Shown are the total cells counted from the three independent transfections. Cells with >5 Flag or 53BP1 foci were scored as Flag or 53BP1 IRIF+, respectively, and cells with ≤5 Flag or 53BP1 foci were scored as IRIF−. The Fisher's exact test was used for statistics.