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. 2014 May 15;42(12):7981–7996. doi: 10.1093/nar/gku424

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Swapping of KSHV miRNA stem-loops or pre-miRNAs. Either miR-K9 (A) or miR-K5 (B) stem-loops were swapped with miR-K6 or miR-K11 stem-loops. Left panel: schematic view of the mutants. The miRNA stem-loops (mutants 1 and 2) or the pre-miRNAs (mutants 3 and 4) are indicated in black and in color. The abundance of the corresponding mature miRNAs is indicated by + or −. Middle panel: northern blot analysis of the accumulation of mature miRNAs, after overexpression of wt and mutant plasmids in HEK293Grip cells (n = 4). miR-K1–5p and cellular miR-16 were probed as a control of expression and as a loading control, respectively. Right panel: histograms showing the relative expression of the different miRNAs from the mutated plasmids compared to the wt. *P < 0.05 and **P < 0.005.