Table 1.
Absolute quantification of KSHV miRNAs from various cell lines. miRNA accumulation is defined by an absolute amount (fmol of miRNA per µg of total RNA) and by a relative percentage of expression.
| DG-75-K10/12 | BCBL-1 | BC-3 | ||||
|---|---|---|---|---|---|---|
| 10−3 fmol/μg | % | 10−3 fmol/μg | % | 10−3 fmol/μg | % | |
| miR-K1–5p | 96.28 ± 3.79 | 5.66 | 114.33 ± 8.16 | 3.03 | 407.48 ± 35.66 | 2.87 |
| miR-K2–5p | 53.58 ± 10.26 | 3.15 | 43.25 ± 2.83 | 1.15 | 90.59 ± 13.23 | 0.64 |
| miR-K3–5p | 389.36 ±98.73 | 22.89 | 307.11 ± 80.96 | 8.15 | 1739.90 ± 394.52 | 12.28 |
| miR-K4–5p | 14.28 ± 3.27 | 0.84 | 64.48 ± 6.38 | 1.71 | 278.06 ± 45.74 | 1.96 |
| miR-K4–3p | 266.03 ± 30.92 | 15.64 | 638.33 ± 94.78 | 16.93 | 2577.42 ± 477.58 | 18.18 |
| miR-K5–3p | 10.68 ± 3.99 | 0.63 | 30.29 ± 7.94 | 0.80 | 78.12 ± 13.06 | 0.55 |
| miR-K6–5p | 60.26 ± 21.36 | 3.54 | 85.22 ± 13.17 | 2.26 | 257.43 ± 68.29 | 1.82 |
| miR-K6–3p | 263.15 ± 44.71 | 15.47 | 451.99 ± 41.72 | 11.99 | 1509.03 ± 279.25 | 10.65 |
| miR-K11–3p | - | 0.00 | 872.40 ± 162.05 | 23.14 | 2003.58 ± 598.64 | 14.14 |
| miR-K7–3p | 312.61 ± 146.44 | 18.38 | 830.43 ± 219.40 | 22.03 | 4978.93 ± 1115.48 | 35.13 |
| miR-K8–3p | 64.61 ± 50.35 | 3.80 | 79.12 ± 19.28 | 2.10 | 253.70 ± 118.76 | 1.79 |
| miR-K9–5p | 121.06 ± 23.94 | 7.12 | 181.12 ± 53.32 | 4.81 | - | 0.00 |
| miR-K9–3p | 49.30 ± 14.01 | 2.90 | 71.24 ± 10.72 | 1.89 | - | 0.00 |
| Total miRNA | 1701.20 | 100.00 | 3769.31 | 100.00 | 14174.24 | 100.00 |