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. 2014 May 21;42(12):8008–8023. doi: 10.1093/nar/gku431

Figure 6.

Figure 6.

Cwc2 W37A suppresses the prp16-302 cold sensitive growth defect at 16°C. (A) Schematic diagram of the Prp16 protein. The seven conserved domains that define DExD/H box proteins are shown in grey. The sequences of these motifs within Prp16 are shown. The prp16-302 mutant carries two mutations. The R456K mutation is located in a highly conserved residue located in between domain Ia and II. The G691R mutation is located in the conserved VI domain. (B) Genetic suppression of prp16-302 by Cwc2 W37A. The plasmid shuffle assay was performed in the CWC2KO/PRP16KO strain containing the wild-type CWC2 and PRP16 genes on a URA3 marked plasmid. The strain was co-transformed with mutant Cwc2 and Prp16 alleles on LEU2 and HIS3 marked plasmids, respectively. One in five serial dilutions of yeast, with an initial OD600 of 1, were grown on plates containing 5-FOA at 16, 25, 30 and 37°C for 3 days, except for strains grown at 16°C which were left for 5 days.