Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 May 28;42(12):8049–8061. doi: 10.1093/nar/gku480

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Analysis of interferon response induction. (A) 400 ng of esiRNAs or 200 ng siRNA pools both against Scyl1, PolG, Traf5 and Ago2 were loaded onto a 20% native PAA gel and stained with ethidium bromide. (B and C) MCF7 cells were transfected with 1, 10 or 30 nM siPools or corresponding esiRNAs against Scyl1 (B) or PolG (C). mRNA levels were normalized to GAPDH and relative expression levels were calculated using a negative control siPool. (D) MCF7 cells were transfected with 10 nM esiRNAs (left) or a siPool containing 60 siRNAs (right) against Scyl1. mRNA expression profiles were assessed using microarray.