Table 1.
Jmjd6-interacting proteins with RS-domain
| Accession number | Name | GFP-tag (IP 1) | GFP-tag (IP 2) | TAP-tag (Webby et al.) | HA-tag (Rahman et al.) | Endogenous interaction | RS domain (uniprot) |
|---|---|---|---|---|---|---|---|
| Q9Y383 | Putative RNA-binding protein Luc7-like 2 | + | + | + | + | + | + |
| Q9NQ29 | Putative RNA-binding protein Luc7-like 1 | + | + | + | + | + | + |
| O95232 | Luc7-like protein 3 | + | + | + | + | + | + |
| Q16630 | Cleavage and polyadenylation specificity factor subunit 6 | + | + | + | + | − | + |
| Q01081 | Splicing factor U2AF 35 kDa subunit | + | + | + | + | − | + |
| P26368 | Splicing factor U2AF 65 kDa subunit | + | + | + | + | + | + |
| Q05519 | Serine/arginine-rich splicing factor (SRSF) 11 | + | + | + | + | + | + |
| Q14498 | RNA-binding protein 39 | + | + | + | + | + | + |
| Q7L4I2 | Arginine/serine-rich coiled-coil protein 2 | + | + | − | + | − | + |
| Q7L014 | Probable ATP-dependent RNA helicase DDX46 | + | + | + | + | − | + |
| Q8WXA9 | Splicing regulatory glutamine/lysine-rich protein 1 | + | + | − | + | − | + |
| Q9UQ35 | Serine/arginine repetitive matrix protein 2 | + | + | − | + | − | + |
| O75400 | Pre-mRNA-processing factor 40 homolog A | + | + | + | + | − | + |
| Q9UKV3 | Apoptotic chromatin condensation inducer in the nucleus (Acinus) | + | + | + | − | − | + |
| Q8N5F7 | NF-kappa-B-activating protein | + | + | + | + | − | + |
| Q96IZ7 | Serine/Arginine-related protein 53 | + | − | + | + | − | + |
| O43809 | Cleavage and polyadenylation specificity factor subunit 5 | + | + | − | + | − | − |
| Q9UHX1 | Poly(U)-binding-splicing factor PUF60 | + | + | + | + | + | − |
| Q15637 | Splicing factor 1 | + | + | − | + | − | − |
| Q13435 | Splicing factor 3B subunit 2 | + | + | − | + | − | − |
| Q9UJV9 | Probable ATP-dependent RNA helicase DDX41 | + | − | + | + | − | − |
| P05455 | Lupus La protein | + | + | − | + | − | − |
| P11142 | Heat shock cognate 71 kDa protein | + | + | − | + | − | − |
| P38646 | Stress-70 protein, mitochondrial | + | + | − | + | − | − |
| P08107 | Heat shock 70 kDa protein 1A/1B | + | + | − | + | − | − |
| P06748 | Nucleophosmin | + | + | − | + | − | − |
| P50990 | T-complex protein 1 subunit theta | + | + | − | + | − | − |
| P50991 | T-complex protein 1 subunit delta | + | + | − | + | − | − |
| Q99832 | T-complex protein 1 subunit eta | + | + | − | + | − | − |
| P10599 | Thioredoxin | + | + | − | + | − | − |
| Q15084 | Protein disulfide-isomerase A6 | + | + | − | + | − | − |
| P62263 | 40S ribosomal protein S14 | + | + | − | + | − | − |
| Q9BU76 | Multiple myeloma tumor-associated protein 2 | + | − | + | + | − | − |
| Q15365 | Poly(rC)-binding protein 1 | + | + | − | + | − | − |
| Q15366 | Poly(rC)-binding protein 2 | + | + | − | + | − | − |
Summary of putative Jmjd6 binding proteins identified in two independent GFP-pulldown assays (IP 1 + 2) coupled to mass spectrometry (LC–MS/MS) after transient expression of Jmjd6-GFP in HEK 293T cells and comparison with recently published Jmjd6 pulldown results using TAP-tagged (8) or HA-tagged Jmjd6 in 293T cells (33). Only such proteins are listed that appeared in at least three of these four independent pulldown experiments. Column ‘endogenous interaction’ indicates Jmjd6–protein interactions that have been confirmed to occur on endogenous level, including this work and (8). Proteins connected to pre-mRNA processing are indicated in bold. Presence of RS-domains as defined by www.uniprot.com and as found by manual inspection is indicated in column ‘RS-domain’.