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. 2014 May 31;42(12):7935–7946. doi: 10.1093/nar/gku494

Figure 1.

Figure 1.

Single-molecule assay for analysis of ScTopA relaxation activity. (A) The scheme of the experiment. (B) Extension versus supercoiling curve for 2-kb dsDNA extended by a force F = 0.3 pN. (C) Time-trace of ScTopA activity acting on 2-kb dsDNA for positive (+SC) and negative (−SC) supercoiling.