Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1995 Oct 24;92(22):10432–10435. doi: 10.1073/pnas.92.22.10432

Transfer RNA editing in land snail mitochondria.

S Yokobori 1, S Pääbo 1
PMCID: PMC40811  PMID: 7479799

Abstract

Some mitochondrial tRNA genes of land snails show mismatches in the acceptor stems predicted from their gene sequences. The majority of these mismatches fall in regions where the tRNA genes overlap with adjacent downstream genes. We have synthesized cDNA from four circularized tRNAs and determined the sequences of the 5' and 3' parts of their acceptor stems. Three of the four tRNAs differ from their corresponding genes at a total of 13 positions, which all fall in the 3' part of the acceptor stems as well as the discriminator bases. The editing events detected involve changes from cytidine, thymidine, and guanosine to adenosine residues, which generally restore base-pairing in the stems. However, in one case an A-A mismatch is created from an A-C mismatch. It is suggested that this form of RNA editing may involve polyadenylylation of the maturing tRNAs as an intermediate.

Full text

PDF

Images in this article

10433Fig. 1
on p.10433
10433Fig. 2
on p.10433
10434Fig. 3
on p.10434

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson S., Bankier A. T., Barrell B. G., de Bruijn M. H., Coulson A. R., Drouin J., Eperon I. C., Nierlich D. P., Roe B. A., Sanger F. Sequence and organization of the human mitochondrial genome. Nature. 1981 Apr 9;290(5806):457–465. doi: 10.1038/290457a0. [DOI] [PubMed] [Google Scholar]
  2. Attardi G. Animal mitochondrial DNA: an extreme example of genetic economy. Int Rev Cytol. 1985;93:93–145. doi: 10.1016/s0074-7696(08)61373-x. [DOI] [PubMed] [Google Scholar]
  3. Boore J. L., Brown W. M. Complete DNA sequence of the mitochondrial genome of the black chiton, Katharina tunicata. Genetics. 1994 Oct;138(2):423–443. doi: 10.1093/genetics/138.2.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bruce A. G., Uhlenbeck O. C. Reactions at the termini of tRNA with T4 RNA ligase. Nucleic Acids Res. 1978 Oct;5(10):3665–3677. doi: 10.1093/nar/5.10.3665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cattaneo R. Different types of messenger RNA editing. Annu Rev Genet. 1991;25:71–88. doi: 10.1146/annurev.ge.25.120191.000443. [DOI] [PubMed] [Google Scholar]
  6. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  7. Clary D. O., Wolstenholme D. R. The mitochondrial DNA molecular of Drosophila yakuba: nucleotide sequence, gene organization, and genetic code. J Mol Evol. 1985;22(3):252–271. doi: 10.1007/BF02099755. [DOI] [PubMed] [Google Scholar]
  8. Gray M. W. Origin and evolution of organelle genomes. Curr Opin Genet Dev. 1993 Dec;3(6):884–890. doi: 10.1016/0959-437x(93)90009-e. [DOI] [PubMed] [Google Scholar]
  9. Hatzoglou E., Rodakis G. C., Lecanidou R. Complete sequence and gene organization of the mitochondrial genome of the land snail Albinaria coerulea. Genetics. 1995 Aug;140(4):1353–1366. doi: 10.1093/genetics/140.4.1353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hoffmann R. J., Boore J. L., Brown W. M. A novel mitochondrial genome organization for the blue mussel, Mytilus edulis. Genetics. 1992 Jun;131(2):397–412. doi: 10.1093/genetics/131.2.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Janke A., Päbo S. Editing of a tRNA anticodon in marsupial mitochondria changes its codon recognition. Nucleic Acids Res. 1993 Apr 11;21(7):1523–1525. doi: 10.1093/nar/21.7.1523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. L'Abbé D., Lang B. F., Desjardins P., Morais R. Histidine tRNA from chicken mitochondria has an uncoded 5'-terminal guanylate residue. J Biol Chem. 1990 Feb 15;265(5):2988–2992. [PubMed] [Google Scholar]
  13. Lecanidou R., Douris V., Rodakis G. C. Novel features of metazoan mtDNA revealed from sequence analysis of three mitochondrial DNA segments of the land snail Albinaria turrita (Gastropoda: Clausiliidae). J Mol Evol. 1994 Apr;38(4):369–382. doi: 10.1007/BF00163154. [DOI] [PubMed] [Google Scholar]
  14. Lonergan K. M., Gray M. W. Editing of transfer RNAs in Acanthamoeba castellanii mitochondria. Science. 1993 Feb 5;259(5096):812–816. doi: 10.1126/science.8430334. [DOI] [PubMed] [Google Scholar]
  15. Maréchal-Drouard L., Ramamonjisoa D., Cosset A., Weil J. H., Dietrich A. Editing corrects mispairing in the acceptor stem of bean and potato mitochondrial phenylalanine transfer RNAs. Nucleic Acids Res. 1993 Oct 25;21(21):4909–4914. doi: 10.1093/nar/21.21.4909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ojala D., Montoya J., Attardi G. tRNA punctuation model of RNA processing in human mitochondria. Nature. 1981 Apr 9;290(5806):470–474. doi: 10.1038/290470a0. [DOI] [PubMed] [Google Scholar]
  17. Okimoto R., Macfarlane J. L., Clary D. O., Wolstenholme D. R. The mitochondrial genomes of two nematodes, Caenorhabditis elegans and Ascaris suum. Genetics. 1992 Mar;130(3):471–498. doi: 10.1093/genetics/130.3.471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Steinberg S., Misch A., Sprinzl M. Compilation of tRNA sequences and sequences of tRNA genes. Nucleic Acids Res. 1993 Jul 1;21(13):3011–3015. doi: 10.1093/nar/21.13.3011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wolstenholme D. R. Animal mitochondrial DNA: structure and evolution. Int Rev Cytol. 1992;141:173–216. doi: 10.1016/s0074-7696(08)62066-5. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES