Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jun 9;42(12):7694–7707. doi: 10.1093/nar/gku503

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — Inactivating mutations of putative phosphorylation and ubiquitination sites predicted within ORF1p sequence abolish MT1 effect on ORF1p. (A) Schematic of putative phosphorylation (ORF1 Ser) and ubiquitination (ORF1 Ub) sites predicted within the ORF1p sequence mutated to alanine. (B) Western blot analysis of ORF1p in the presence or absence of melatonin receptor 1 expression (MT1 and control, respectively). Transfection with an empty vector was used to assess background levels of ORF1p. GAPDH is used as loading control. (C) Quantitation of western blot results (N = 3). ORF1, ORF1 Ser and ORF1 Ub expression in the presence of MT1 (black bars) is normalized to their respective expression in the presence of control vector (gray bars). Error bars are standard deviation; asterisks indicate statistically significant differences between control and MT1 lanes by t-test.