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. 2014 Jun 16;42(12):8174–8187. doi: 10.1093/nar/gku512

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Comparison of exon skipping activity of short (6- to 9-mer) LNA SSOs. (A) Schematic representation of the position of LNA in the SSOs used in this study. (B) The reporter cells were transfected with the indicated LNA/DNA mixmer SSOs (30 nM) for 24 h. RT-PCR analyses were performed as described in Figure 3B. (C) The levels of exon 58-skipped mRNA fragments were measured by quantitative real-time RT-PCR (for details see Materials and Methods and Figure 2G). Values represent the mean ± standard deviation of triplicate samples. Reproducible results were obtained from two independent experiments. The T_m of each SSO with a complementary RNA under low-sodium conditions is also shown. The data are the mean ± standard deviation (n = 3–4).