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Exon skipping by LNA SSOs in primary human skeletal muscle cells. HSMM cells were transfected with the indicated LNA/DNA mixmer SSOs (500 nM) for 24 h. RT-PCR analyses show the full-length upper band (575 bp) and the skipped lower band (454 bp). LNA SSO (+10+24), which showed no exon skipping effects, was used as a control. GAPDH was used as an internal control.
