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. 2014 Jul 3;9(7):e98197. doi: 10.1371/journal.pone.0098197

Table 1. Pcdh1 RACE and qPCR primer sequences.

Pcdh1 RACE primer Sequence (5′-3′) Corresponding GeneRacer primer Sequence (5′-3′)
5′RACE 301Rev GAGGTCTCTGTGGTGAAAATGTCT GeneRacer 5′ primer CGACTGGAGCACGAGGACACTGA
5′RACE 162Rev GTTCCTCTGGCACCTTGTATACTACC GeneRacer 5′ nested primer GGACACTGACATGGACTGAAGGAGTA
3′RACE FW1 GCCCAGGAGCTGCAGGATCCAT GeneRacer 3′ primer GTCGTCAACGATACGCTACGTAACG
3′RACE FW2 GAAACACCCTCTAGCAAGTCATCCT GeneRacer 3′ nested primer CGCTACGTAACGGCATGACAGTG
5′RACE Ex3 Rev3 GCTGTGATGGATCCTGCAGCTCCT GeneRacer 5′ primer CGACTGGAGCACGAGGACACTGA
5′RACE Ex3 Rev2 ACTGGTGGCCGAGAAGGTGACA GeneRacer 5′ nested primer GGACACTGACATGGACTGAAGGAGTA
qPCR assay Forward (5′–3′) Probe (FAM-NFQ labelled) Reverse (5′-3′)
M-1B–Ex1 CTCAGCATGCGCAGAAGAAAA ACTGTTCTCCTGATTCTGG CCTCAGAGGCCCCATCCT
Ex2-Ex3 GAGCAGTACTCCGACTACAG CCAGCAAGCAGTTACCTCAC TAGAGGGTGTTTCCGATTCC

Ex = exon; Rev = reverse; FW = Forward.