Table 1. Pcdh1 RACE and qPCR primer sequences.
Pcdh1 RACE primer | Sequence (5′-3′) | Corresponding GeneRacer primer | Sequence (5′-3′) |
5′RACE 301Rev | GAGGTCTCTGTGGTGAAAATGTCT | GeneRacer 5′ primer | CGACTGGAGCACGAGGACACTGA |
5′RACE 162Rev | GTTCCTCTGGCACCTTGTATACTACC | GeneRacer 5′ nested primer | GGACACTGACATGGACTGAAGGAGTA |
3′RACE FW1 | GCCCAGGAGCTGCAGGATCCAT | GeneRacer 3′ primer | GTCGTCAACGATACGCTACGTAACG |
3′RACE FW2 | GAAACACCCTCTAGCAAGTCATCCT | GeneRacer 3′ nested primer | CGCTACGTAACGGCATGACAGTG |
5′RACE Ex3 Rev3 | GCTGTGATGGATCCTGCAGCTCCT | GeneRacer 5′ primer | CGACTGGAGCACGAGGACACTGA |
5′RACE Ex3 Rev2 | ACTGGTGGCCGAGAAGGTGACA | GeneRacer 5′ nested primer | GGACACTGACATGGACTGAAGGAGTA |
qPCR assay | Forward (5′–3′) | Probe (FAM-NFQ labelled) | Reverse (5′-3′) |
M-1B–Ex1 | CTCAGCATGCGCAGAAGAAAA | ACTGTTCTCCTGATTCTGG | CCTCAGAGGCCCCATCCT |
Ex2-Ex3 | GAGCAGTACTCCGACTACAG | CCAGCAAGCAGTTACCTCAC | TAGAGGGTGTTTCCGATTCC |
Ex = exon; Rev = reverse; FW = Forward.