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Enzyme specificity. The enzymes were incubated with the following substrates each at 1.0 mM: mevalonate (MVA), mevalonate 5-phosphate (M5P), mevalonate 5-pyrophosphate (MPP), mevalonate 3-phosphate (M3P), and 2-phosphoglycerate (2PG). A no enzyme control (NE) was also performed. ATP (1.0 mM) was included, and ADP production was monitored at 55 °C and pH 8.5 for Ta1305 and 60 °C and pH 8.0 for Ta0762.
