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13C NMR of completed enzymatic reactions (10 mM products) in 50% D2O. The chemical shifts of the six mevalonate carbons are listed along with the J couplings from 31P (red): (A) no enzyme, (B) yeast mevalonate-5-kinase, (C) Ta1305 activity, and (D) Ta1305 and Ta0762 activity. Reactions were monitored to >96% completion before spectra were acquired. Full spectra are shown in Figures S2–S4 and S6 of the Supporting Information.
