Table I.
The mutation frequencies of K-ras gene in different sample types, genders and age groups of lung cancer patients detected with COLD-PCR and sequencing.
| Mutation frequency (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
|
|
|||||||||
| Type of samples | Gender | Age (years) | |||||||
|
|
|
|
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| Amino acid change | K-ras mutation | Plasma N=51 |
Tumor tissue N=131 |
Pleural and ascites N=5 |
Male N=123 |
Female N=64 |
Youth (25–44) N=9 |
Middle age (45–59) N=72 |
Elderly (60–90) N=106 |
| G12S | GGT>AGT | ||||||||
| G12R | GGT>CGT | ||||||||
| G12C | GGT>TGT | 2 (1.53) | 2 (1.63) | 1 (1.39) | 1 (0.94) | ||||
| G12D | GGT>GAT | 5 (3.82) | 1 (20) | 4 (3.25) | 2 (3.13) | 1 (1.39) | 5 (4.72) | ||
| G12A | GGT>GCT | ||||||||
| G12V | GGT>GTT | 4 (3.05) | 2 (1.63) | 2 (3.13) | 1 (1.39) | 3 (2.83) | |||
| G13S | GGC>AGC | ||||||||
| G13R | GGC>CGC | ||||||||
| G13C | GGC>TGC | ||||||||
| G13D | GGC>GAC | 1 (1.96) | 4 (3.05) | 3 (2.44) | 2 (3.13) | 1 (11.11) | 2 (2.78) | 2 (1.89) | |
| G13A | GGC>GCC | ||||||||
| G13V | GGC>GTC | ||||||||
| Q61K | CAA>AAA | ||||||||
| Q61L | CAA>CTA | ||||||||
| Q61H | CAA>CAT | ||||||||
| Total (%) | 1.96 | 11.45 | 20 | 8.94 | 9.38 | 11.11 | 6.94 | 10.38 | |