Table II.
The mutation frequencies of K-ras gene in different sample types, genders, and age groups of colorectal cancer patients detected with COLD-PCR and sequencing.
| Mutation frequency | |||||||||
|---|---|---|---|---|---|---|---|---|---|
|
|
|||||||||
| Type of samples | Gender | Age (years) | |||||||
|
|
|
|
|||||||
| Amino acid change | K-ras mutation | Plasma N=60 |
Tumor tissue N=445 |
Pleural and ascites N=0 |
Male N=294 |
Female N=211 |
Youth (25–44) N=60 |
Middle age (45–59) N=195 |
Elderly (60–90) N=250 |
| G12S | GGT>AGT | 2 (0.45) | 2 (0.95) | 1 (0.51) | 1 (0.4) | ||||
| G12R | GGT>CGT | 1 (0.22) | 1 (0.34) | 1 (0.4) | |||||
| G12C | GGT>TGT | 5 (1.12) | 3 (1.02) | 2 (0.95) | 1 (0.51) | 4 (1.6) | |||
| G12D | GGT>GAT | 1 (1.67) | 35 (7.87) | 14 (4.76) | 22 (10.43) | 3 (5) | 12 (6.15) | 21 (8.4) | |
| G12A | GGT>GCT | 4 (0.90) | 3 (1.02) | 1 (0.47) | 2 (1.03) | 2 (0.8) | |||
| G12V | GGT>GTT | 1 (1.67) | 14 (3.15) | 9 (3.06) | 6 (2.84) | 8 (4.10) | 7 (2.8) | ||
| G13S | GGC>AGC | 2 (0.45) | 2 (0.95) | 2 (0.8) | |||||
| G13R | GGC>CGC | 1 (0.22) | 1 (0.34) | 1 (0.51) | |||||
| G13C | GGC>TGC | 1 (0.22) | 1 (0.34) | 1 (0.4) | |||||
| G13D | GGC>GAC | 1 (1.67) | 24 (5.39) | 11 (3.74) | 14 (6.64) | 2 (3.33) | 15 (7.69) | 8 (3.2) | |
| G13A | GGC>GCC | ||||||||
| G13V | GGC>GTC | ||||||||
| Q61K | CAA>AAA | ||||||||
| Q61L | CAA>CTA | 2 (0.45) | 1 (0.34) | 1 (0.47) | 1 (0.51) | 1 (0.4) | |||
| Q61H | CAA>CAT | ||||||||
| Total (%) | 5 | 20.45 | 14.97 | 23.70 | 8.33 | 21.03 | 19.2 | ||