Table III.
The mutation frequencies of K-ras gene in different sample types, genders, and age groups of gastric cancer patients detected with COLD-PCR and sequencing.
| Mutation frequency | |||||||||
|---|---|---|---|---|---|---|---|---|---|
|
|
|||||||||
| Type of samples | Gender | Age (years) | |||||||
|
|
|
|
|||||||
| Amino acid change | K-ras mutation | Plasma N=9 |
Tumor tissue N=126 |
Pleural and ascites N=0 |
Male N=92 |
Female N=43 |
Youth (25–44) N=22 |
Middle age (45–59) N=61 |
Elderly (60–90) N=52 |
| G12S | GGT>AGT | ||||||||
| G12R | GGT>CGT | ||||||||
| G12C | GGT>TGT | ||||||||
| G12D | GGT>GAT | 3 (2.38) | 3 (3.26) | 1 (4.55) | 1 (1.64) | 1 (1.92) | |||
| G12A | GGT>GCT | ||||||||
| G12V | GGT>GTT | ||||||||
| G13S | GGC>AGC | ||||||||
| G13R | GGC>CGC | ||||||||
| G13C | GGC>TGC | ||||||||
| G13D | GGC>GAC | 6 (4.76) | 4 (4.35) | 2(4.65) | 1 (4.55) | 2 (3.28) | 3 (5.77) | ||
| G13A | GGC>GCC | ||||||||
| G13V | GGC>GTC | ||||||||
| Q61K | CAA>AAA | ||||||||
| Q61L | CAA>CTA | ||||||||
| Q61H | CAA>CAT | ||||||||
| Total (%) | 0 | 7.14 | 7.61 | 4.65 | 9.09 | 4.92 | 7.69 | ||