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. 1999 Mar 1;103(5):731–738. doi: 10.1172/JCI653

Figure 3.

Figure 3

SDS-PAGE of type I collagen (soluble calf skin collagen) after digestion with recombinant wild-type and Y212C cathepsin K enzymes. (a) Collagenase activity. Digestion of soluble calf skin collagen at 28°C and pH 5.5 for 12 h by wild-type and Y212C cathepsin K enzymes is shown. The presence (+) or absence (–) of collagen and the concentration of the cathepsin K in nM are indicated. (b) Gelatinase activity. Digestion of denatured soluble calf skin collagen by wild-type and Y212C cathepsin K enzymes is shown. The presence (+) or absence (–) of gelatin and the concentration of the cathepsin K in nM are indicated. Molecular mass standards (measured in kDa) are indicated in the right margin, and the collagen proteins are labeled in the left margin.