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Apoptosis of fibroblasts subjected to thi– medium. TUNEL stain was performed as described in Methods. Normal (a and b) or TRMA mutant cells (c and d) were incubated for 10 days in the presence (a and c) or absence (b and d) of thiamine. (a) Wild-type cells, thiamine-replete. (b) Wild-type cells, thiamine-depleted. (c) TRMA mutant cells with thiamine. (d) TRMA fibroblasts, thiamine-depleted. ×40. TUNEL, deoxynucleotide transferase–mediated dUTP nick end-labeling.
