Table 1. Primers used for real-time quantitative PCR of the EPS-related genes and 16S rRNA in L. fermentum TDS030603.

Gene	Primer	Sequence (5’→3’)	Tm (°C)	Product size (bp)
epsB	BF2 sense	GAAGGTAAGCACCCAAACCA	60.0	234
	BR2 antisense	ATCAAGAACCCAAGCACCAA	60.5
epsC	CF2 sense	ATCCGGACCAACATTCACTT	59.3	214
	CR2 antisense	TGGTTAACCCCTTTTGGTTG	59.7
epsD	DF2 sense	TATTCTGGAGGCGTTTTTGG	60.1	205
	DR2 antisense	AAACTCACGGGCCATTTTT	59.4
epsE	EF2 sense	CGAGTAGGCCGTAATGGAAA	60.1	209
	ER2 antisense	GGTCGTGGACCAATAAAGGA	59.8
epsF	FF2 sense	TCAAGCGGGTATTTGACTTCTT	60.1	238
	FR2 antisense	AACATTGGGCCATCAACATC	60.6
epsG	GF2 sense	GTTGGCCTTATGGAAGCAGA	60.2	158
	GR2 antisense	CAATTGAATCCCGAGTGAAAG	59.6
16S rRNA	16SF1 sense	GCGTTATCCGGATTTATTGG	59.3	240
	16SR1 antisense	CTGTTCGCTACCCATGCTTT	60.3