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Sedimentation velocity analysis of HC, tapasin, and TAP in the microsomes of H39 and KMW-B2 cells. The digitonin-solubilized microsomes were analyzed by glycerol density gradient centrifugation as described in Methods. After centrifugation, the gradient was separated into 34 fractions of 1 ml each. Samples of the fractions were subjected to Western blot analysis. HC, heavy chain; Ab, antibody.
