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JAK2 V617F mutation detected by allele-specific polymerase chain reaction. Lanes; 1, molecular weight marker (100–1,500 bp); 2, JAK2 V617F-positive control (203 bp); 3, JAK2 V617F mutation allele (case 1); 4, wild-type allele for JAK2 (364 bp, case 1); 5, JAK2 V617F mutation allele (case 2); 6, wild-type allele for JAK2 (364 bp, case 2); and 7, negative control.
