Figure 5.

ES7 inhibits later stages of cell plate maturation. In the early steps of cell plate formation, YFP-RABA2A (A), KNOLLE-GFP (B), and DRP1C-GFP (C) accumulate at the center of the dividing cell. Early-stage cell plates labeled by these markers are not affected by ES7 (D–F). During later stages of cell plate maturation, YFP-RABA2A largely accumulates at the edge of the growing cell plate (G), KNOLLE-GFP and DRP1C-GFP localize along the cell plate (H and I), and CLC2-GFP shows relatively low levels of cell plate accumulation while localizing in the vicinity of the cell plate (J). During later maturation stages, ES7 causes split cell plates with abnormal accumulation of the markers (K–N). Note the aggregation of KNOLLE-GFP at cell plate stubs (L). FM4-64 counterstaining of the plasma membrane is indicated by magenta, and the respective markers are indicated in green. Bars = 10 μm.