Figure 7.

ES7 inhibits cell plate maturation after FVS. A, C, and E, Vesicle accumulation during the FVS is not affected by ES7. B, D, F, and G, A 2-h treatment with 50 µm ES7 abolished callose deposition at the cell plate, arresting cell plate maturation. This is indicated by the absence of the fluorescent markers in the center of the plate (arrowheads), normally the site of callose deposition. Note the aggregation of KNOLLE (D). No cell plate localization of CLC2-GFP is detectable (G). α-Callose is marked in magenta, while the respective fluorescent markers are indicated in green. A.U., Arbitrary units. Bars = 10 µm.