Figure 2.

Combination mutants with defects in IBA metabolism and IAA-conjugate hydrolysis have germination delays. A and B, Wild-type and ill2 iar3 ibr3 (A) or ill2 ilr1 iar3 ibr1 (B) germination was measured on media with no hormone, 3 µm IBA, or 20 nm IAA. Data points are the averages from three germination experiments. Values on d 2 to 4 for the mutants on no hormone or IBA are significantly different than corresponding lines grown on 20 nm IAA or the wild type (P < 0.05). C, IAA rescues the ill2 iar3 ibr3 germination defect in a concentration-dependent manner. D, The percentage of plants germinated by d 3 for the wild type and the ill2 iar3 ibr3 mutant on no hormone or increasing concentrations of IAA, IBA, IAA-Ala, and IAA-Leu. n = 3; error bars represent the se, and a to c indicate significant differences using one-way ANOVA (P < 0.05). Wt, Wild type.